Efficient protocol improved the yield and viability of oil palm protoplasts isolated from in vitro leaf and mesocarp

•An efficient protocol for protoplasts isolation from in vitro leaf and mesocarp of oil palm has been established.•Enzymes composition effect on the yield and viability of protoplast are significant.•Scraping the waxy layer on oil palm leaf increased the protoplast isolation efficiency.•Vacuum treatment enhanced the penetration of enzymes into tissues.•10% ficoll in 0.6 M mannitol improved the separation of protoplasts from impurities.