Comparative Study On Conventional And Auto-Induction Fermentation

The biosynthesis of recombinant proteins with non-native structures and expressed in a non-soluble state is a major challenge. More than 30% of the recombinant protein produced by the most commonly used prokaryotic expression system (Escherichia coli) is produced in an insoluble form, further steps are required to solubilize it.In our experiments, we compared the inducer effect, the monitored growth parameters of the conventional broth (LB, 2YT), and the auto-induction broth.