Michael addition reaction of symmetrically halogenated bischalcones with reduced glutathione assessed by RP-HPLC and RP-HPLC-ESI-MS

Livia Oliveira Alves Ferreira,Szilard Molnar,Felipe Terra Martins,Pal Perjesi,Luiz Henrique Keng Queiroz,Caridad Noda-Perez

Microchemical Journal（2021）

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摘要

•Michael's reaction of reduced glutathione to symmetric halogenated bischalcones.•Difference between compounds formed in PEG400 and in methanol.•Adduct formed by the addition of a GSH molecule to the second double bond of SHBC's.•Study of the compounds formed by RP-HPLC-ESI-MS.•Determination of GSH and GSSG concentration during the PEG400 reaction.

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关键词

Michael addition reaction,Symmetrically halogenated bischalcones (SHBCs),Reduced glutathione (GSH),RP-HPLC analysis,RP-HPLC-ESI-MS analysis

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