Effects Of Microrna-203 On Farage Cell Apoptosis In Diffuse Large B Cell Lymphoma

Survivin is a most potent member in apoptotic inhibitor protein family, and exerts anti-apoptotic effect via inhibiting caspase-3 activity. Study has found significantly elevated Survivin expression in disuse large B cell lymphoma (DLBCL) patients. Meanwhile, remarkably decreased microRNA (miR)-203 expression was observed in B lymphocytes of DLBCL patients, indicating its tumor suppressing role. Bioinformatics analysis showed satisfactory targeting relationship between miR-203 and 3'-UTR of Survivin mRNA. This study thus investigated whether miR-203 played a role in regulating Survivin expression and affecting apoptosis of DLBCL cell line Farage. A total of 38 DLBCL patients in our hospital were recruited to collect tumor samples, in parallel with 46 lymph tissues samples with reactive hyperplasia as the control group. MiR-203 and Survivin expression were tested, and their targeted relationship was assessed by dual luciferase reporter gene assay. In vitro cultured Farage cells were divided into mimic NC group, miR-203 mimic group, si-NC group, si-Survivin group and miR-203 mimic + si-Survivin group. Spectrometry was used to test caspase-3 activity, while flow cytometry was utilized for analysis of cell apoptosis, followed by Western blot analysis of protein expressions. Compared with control group, DLBCL tissues had significantly lowered miR-203 expression, plus higher Survivin expression. MiR-203 targeted 3'-UTR of Survivin mRNA and inhibited its expression. Elevation of miR-203 and/or silencing of Survivin expression significantly potentiated Caspase-3 activity and facilitated Farage cell apoptosis. MiR-203 inhibited Survivin expression via targeted inhibition, thus depressing its inhibitor effect on Caspase-3 activity, leading to accelerated Farage cell apoptosis.