Cytogenetic Studies On The Social Parasite Acromyrmex Ameliae (Formicidae: Myrmicinae: Attini) And Its Hosts Reveal Chromosome Fusion In Acromyrmex

The ant Acromyrmex ameliae is a social parasite of two leaf-cutting ant subspecies: Acromyrmex subterraneus subterraneus and A. subterraneus brunneus. Cytogenetic data are available for 14 species of Acromyrmex and all of them possess 2n = 38 chromosomes. In this study, chromosome number, heterochromatin detection, and detection of AT and GC-rich blocks of colonies of A. ameliae and its hosts were carried out. Additionally, the detection of nucleolus organizer regions and 18S rDNA clusters in chromosomes of the parasite and physical mapping of telomeres were undertaken. The same chromosome number and morphology were detected for the hosts 2n = 38 (10m + 14sm + 12st + 2a), while the females and males of the social parasite A. ameliae presented 2n = 36 (10m + 16sm + 8st + 2a) and n = 18 (5m + 8sm + 4st + 1a). In both A. ameliae and its hosts, the terminal region on the short arm of the largest subtelocentric pair is heterochromatic GC-rich, and this region corresponded to the 18S rDNA clusters in the parasite. The short arms of several chromosomes were heterochromatin-rich. The telomeric probe hybridized telomeres on all chromosomes of the parasite and was not detected in intrachromosomal regions. Through a comparative cytogenetic analysis, we hypothesize that the karyotype of A. ameliae (2n = 36) originated from a chromosomal rearrangement that reduced the number of chromosomes from 38 to 36; as available data on the genus Acromyrmex show that all other species possess 38 chromosomes, representing 45% of the 33 valid species in this genus. The mechanism of the chromosome rearrangement is discussed. Thus, the chromosome number observed in A. ameliae is a derivation from the genus. Our data show variation in the chromosomal number in Acromyrmex and suggest that analyses of the karyotypes of parasite species can yield novel insights with regards to the evolution of this genus. (c) 2021 Elsevier GmbH. All rights reserved.