Transcriptome Analysis Of Fuzheng Xiaoji Prescription Inhibiting The Proliferation Of Colorectal Cancer

This study aimed to explore the potential molecular mechanisms of FuZheng XiaoJi prescription (FZXJP) on Colorectal Cancer (CRC) cells. Transcriptome sequencing was performed on HT-29 cells treated with FZXJP and not followed by selecting differentially expressed genes (DEGs). Functional enrichment analysis and protein-protein interaction (PPI) analysis were performed for the DEGs. The transcription factors were predicted using TRRUST and miRWalk 3.0. Finally, Real-time PCR (qRT-PCR) was used to verify the results of transcriptome sequencing. HT-29 had a more sensitive response to FZXJP. Transcriptome sequencing revealed 1522 DEGs, and they were enriched in various biological processes and pathways, such as regulation of cellular biosynthetic process and MAPK signaling pathway. Totally 61 proteins in the PPI network with degree > 5 were screened as hub genes, including EGR1, KLHL11, UBA7, IRF1, HERC6, and IFI35. The qRT-PCR confirmed that the expression of those genes was consistent with that of transcriptome analysis. Three transcription factors and 15 miRNAs were predicted. All the three transcription factors were found to interact with EGR1. IRF1 was a target of miR-93-5p. This study provided a theoretical basis for the regulation of FZXJP in HT-29cells, which lays a foundation for the treatment of CRC.