Solation And Purification Of Glutamic Acid Decarboxylase From Mung Bean

FRESENIUS ENVIRONMENTAL BULLETIN(2020)

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摘要
gamma-aminobutyric acid (GABA) is a non-protein amino acid with several important physiological functions such as neurotransmission. induction of hypotensive effects, and tranquilizes effects. GABA is produced primarily by the decarboxylation of L-glutamic acid (Glu) catalyzed by glutamate decarboxylase (GAD) [EC4.1.1.15]. Mung bean was an effective catalyst for GABA production with relative high GAD activities. In this paper, GAD were extracted and purified from mung bean, and some researches on their structure and characters were taken. The purpose was to know something about the regulate mechanism of mung bean GAD and to give some help for GABA accumulation,
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关键词
gamma-aminobutyric acid, Mung bean, Glutamic decarboxylase, Separation and purification
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