Effects Of Medium Addition On Ovule Enlargement Of Watermelon Non-Pollinated Ovary

In order to investigate the effect of the concentration of basic medium, Thidiazuron (TDZ), Naphthaleneacetic Acid(NAA), 6-Benzylaminopurine (BA), and kinetin (KT) on gynogenetic development of non-pollinated ovary, the explants of watermelon with four different genotypes of F1 generation explants were chosen as the material of orthogonal test. The results showed that the basic medium and TDZ concentration have significant effect on ovule enlargement rate and they are the main factors of inducing the isolated gynogenetic development of watermelon. When choosing M2 basic medium and its concentration of TDZ from 0.4 mg.L-1 to 0.8 mg.L-1, the induction rate of ovule enlargement is highest, reaching 66.83%. Subsequently, the induction effect of ovule enlargement was the best when the concentration of NAA was 0.5 mg.L-1. When the concentration of NAA was 0.5 mg.L-1, the highest rate of ovule enlargement reached 49.33%. The concentration of BA was different due to the difference in explants, the induction effects of ZhongkeNo.6 and XinongNo.8 are the best. The concentration of BA was 1.5 mg.L-1, and ZhongkeNo.6 and XinongNo.8 ovule enlargement rate reached 59.56 and 41.95%. While the concentration reached 1.5 mg.L-1, the rate of ovule enlargement in ZhengkangNo. 2 and Zhengkangjufeng was 55.50 and 52.90%, respectively. No addition or addition of low concentration of KT is beneficial to the enlargement of ovules. The test results significantly improved the watermelon non-pollinated ovary ovule enlargement rate, which provides strong technical support for success of watermelon non-pollinated ovary culture. (C) 2018 Friends Science Publishers