Quantitative Method Study On Transport Mechanism Of Puerarin, Berberine Hydrochloride And Paeoniflorin

To study the quantitative method evaluated drug transportation across cell membrane. Combined with Fick's law of passive diffusion and Michaelis-Menten equation, with the application of law of mass conservation, to derive equation of drug transportation rate. The overall description for membrane-transportation rate was depicted as the sum of passive diffusion rate and carrier-mediated diffusion rate which were calculated by transportation mass being divided respectively. Puerarin, berberine hydrochloride and paeoniflorin, set as model drugs. The cell membrane transportations for these three drugs were anlysized by fitting into this transportation rate theory on different drug concentration and drug combination to figure out the main biotransportation manner. Transportation rates of model drugs were obtained. Compared with the overall transportation manner, the passive diffusion and carrier-mediated transport were 58.2% and -41.8% respectively of puerarin (167.6 mu g . mL-1), 73.2% and -26.8% respectively for berberine hydrochloride (98.2 mu g . mL-1), 57.7% and -42.3% respectively for paeoniflorin (153.9 mu g . mL-1). At the present of Verapamil or probenecid, the overall transportation rate of puerarin and berberine hydrochloride increased and their ratios converted to positive direction. For paeoniflorin, carrier-mediated transport ratio was changed to positive direction only. The transportation mechanism and the efflux proteins of the three drugs were quantitatively evaluated by the established quantitative methods.