Downregulation Of Cd147 Inhibits Skin Squamous Cell Carcinoma Cell Proliferation, Migration, And Invasion By Inhibiting Phosphorylation Of Erk

Purpose: The aim of the current study was to investigate the effects of CD147 on skin squamous cell carcinoma cells, examining related mechanisms. Methods: Normal human keratinocyte cell line (HaCaT) and human skin squamous cell carcinoma cell lines (HSC-5, SCL-1, and A431) were cultured. HSC-5 cells were transfected by CD147 siRNA (CD147-siRNA group). The siRNA-Negative Control group and Blank group were set. Normal HSC-5 cells and cells of the CD147-siRNA group were cultured in 1640 medium containing 100 mu mol/L U0126. They were named the U0126 group and CD147-siRNA + U0126 group, respectively. Moreover, qRT-PCR was used to detect CD147 mRNA expression levels. Western blotting was performed to detect CD147, ERK, and p-ERK protein expression levels. MTT assays, cell scratch tests, and Transwell experimentation were used to detect cell proliferation, migration, and invasion. Results: CD147 relative expression levels in skin squamous cell carcinoma cells were significantly upregulated (P < 0.01). Compared with the Blank group and siRNA-Negative Control group, p-ERK expression, as well as HSC-5 cell proliferation, migration, and invasion, were declined in the CD147-siRNA group, U0126 group, and CD147-siRNA + U0126 group (P < 0.05). Compared to the CD147-siRNA group and U0126 group, p-ERK expression, as well as HSC-5 cell proliferation, migration, and invasion, were decreased in the CD147-siRNA + U0126 group (P < 0.05). Conclusion: Downregulation of CD147 may inhibit proliferation, migration, and invasion of skin squamous cell carcinoma cells by inhibiting phosphorylation of ERK.