Hplc Determination Of Methionine, Homocysteine And Cysteine Enantiomers In Serum Of Patients After Stroke

Homocysteine is sulfur-containing amino acid that is naturally occurring in human body. It is produced from essential amino acid, methionine. Homocysteine can be remethylated back to methionine or converted to cysteine. The association between these amino acids evokes the need for their simultaneous determination. There are many studies focusing on achiral separations. In this work, chiral separations of homocysteine, cysteine and methionine enantiomers are presented. Their concentrations were estimated by using two-dimensional HPLC system with electrochemical detection. The amino acids were separated on achiral column and their enantiomers on Chirobiotic TAG column. The proposed method was applied to the analysis of human serum of healthy volunteers and patients after stroke.