Design Of Secretion Vector Constructs For Granzyme H Expression

Granzyme H belongs to the family of serine proteinases, which plays an important role as part of the natural immune system. This enzyme is produced by natural killer cells and the T lymphocytes, inducing apoptosis in target cells. The precise role of the enzyme is not yet clarified, its crystal structure is unknown. Granzyme H was cloned by RT-PCR from total RNA isolated from human lymphocytes. We designed several vector constructs for the optimal expression of Granzyme H: pET20bGraH, pETM50GraH, pETM52GraH. The enzyme produced using these vectors will be more suitable for activity measurements in the future.