Serum Metabonomic Analysis Of Rats With Cisplatin-Induced Nephrotoxicity And Panax Notoginseng Saponins Treatment

Previously, we reported that the Panax Notoginseng Saponins (PNS) had protection against cisplatin-induced nephrotoxicity. Here, we aimed to metabonomically analyze the serum samples from rats with early stage of cisplatin-induced nephrotoxicity and PNS treatment. The rats underwent intraperitoneal injection with a single dose of cisplatin, and a subset of rats underwent intraperitoneal injection with PNS once a day. After 24 hours, the renal function was evaluated, the pathological changes of renal tissue were examined, and the serum samples were analyzed using H-1 nuclear magnetic resonance-based metabonomics technology. The results showed that PNS protected against cisplatin-induced nephrotoxicity. In additional, PCA, PLS-DA and OPLS-DA scores plots of serum samples showed that the normal control group was clearly separated from the cisplatin-only group and the cisplatin-PNS group, while there was a part overlap between the cisplatin-only group and the cisplatin-PNS group. Moreover, the expression of acetone, n-acetyl glycoprotein signals, pyruvate, lipid and VLDL was lower, while the expression of glucose, valine and tyrosine was higher in the cisplatin-only group and the cisplatin-PNS group than that in the normal control group. Furthermore, lactate expression was higher in the cisplatin-only group than that in the cisplatin-PNS group. Phenylalanine expression was higher while tyrosine expression was lower in the cisplatin-PNS group and the normal control group when compared with the cisplatin-only group. Taken together, these results confirmed that the metabolism of cisplatin-induced nephrotoxicity had a huge change and PNS would be an effective medicine. Its protective mechanism may be associated with correcting the disturbed metabolism of cisplatin-induced nephrotoxicity. And here is the first demonstration about it.