Baicalein Inhibits The Invasion, Migration And Epithelial-Mesenchymal Transition Of Bgc-823 Cells Through Nf-Kappa B/Snail Signaling Pathway

Baicalin is one of the main bioactive flavone glucuronides, which is considered as a medical herb with anticancer activity. However, no detailed studies have ever been reported on its anticancer action through NF-kappa B/Snail signaling pathway. This study aimed to investigate the effects of baicalin on the invasion and migration of gastric cancer BGC-823 cells and epithelial-mesenchymal transition (EMT). Different concentrations of baicalein were used to treat with BGC-823 cells. MTT colorimetry was used to observe the inhibitory rate of cell proliferation. The effects of baicalein on the invasion and migration ability of gastric cancer BGC-823 cells were determined by cell invasion and migration assay. The roles of baicalein on EMT of BGC-823 cells were investigated by examining the protein levels of EMT related markers in BGC-823 cells after baicalein and/or TGF-beta 1 treatments by western blot. Finally, the role of baicalein in TGF-beta 1-mediated activation of NF-kappa B/Snail signaling pathway was investigated by examining the protein levels of the factors involved in NF-kappa B/Snail signaling pathway in BGC-823 cells after baicalein and/or TGF-beta 1 treatment by western blot. Our results have shown that baicalein not only inhibited the proliferation of BGC-823 cells, but also reduced the invasion and migration ability of those cells. Baicalein also inhibited EMT of BGC-823 cells and TGF-beta 1-mediated activation of NF-kappa B/Snail signaling pathway by regulating the expression of the related factors. As a result, baicalein could inhibit EMT and suppress the invasion and migration of human gastric cancer BGC-823 cells through NF-kappa B/Snail signaling pathway.