Circkif4a Regulates The Progression Of Gastric Carcinoma By Sponging Mir-135b

Growing evidence shows that circRNA acts as an miRNA sponge to regulate a variety of tumor types. Gastric carcinoma is one of the most malignant cancers. However, the functions of circRNA and its associated regulatory mechanisms remain largely unknown. In this study, we used quantitative real-time PCR (qRT-PCR) to determine that the circKIF4A expression is abnormal in gastric carcinoma tissues and different tumor cell types. Next, we investigated the circKIF4A function in gastric carcinoma using apoptosis and migration assays. Western blot was used to explore the miR-135b sponging function of circKIF4A in gastric carcinoma. A qRT-PCR demonstrated that circKIF4A is up-regulated in gastric carcinoma. The knockdown of circKIF4A promotes apoptosis and the migration of SGC-7901. Moreover, miR-135b regulates the expression of mRNA (including PARP, Pax-4, FOXJ2, and Elf-1) in gastric carcinoma, and circKIF4A inhibits miR-135b's regulatory function on mRNA (including PARP, Pax-4, FOXJ2, and Elf-1). Taken together, our findings show that circKIF4A acts as an miR-135b sponge, which affects the expression of mRNA (including PARP, Pax-4, FOXJ2, and Elf-1) and ultimately acts as a gastric carcinoma promoter.