Regional Distribution Of Guanine-Nucleotide Binding-Proteins (Gs And Gi-Alpha) In Human Brain - Correlation With Adenylyl Cyclase Activity

G(s) and G(i) alpha immunoreactivities and adenylyl cyclase activity were measured in autopsied human brain. Highest levels of G(s) 52 kDa and G(i) alpha subunits occurred in frontal cortex, and highest G(s) 45 kDa levels in caudate. Although basal adenylyl cyclase activity was similar in all regions, GTPgammaS-stimulated adenylyl cyclase activity and forskolin-stimulated activity showed specific brain regional patterns which correlated with G(s) 45 kDa and G(i) alpha levels underscoring an important functional relationship between these measures.