Mechanism Underlying The Regulation Of Trophoblast-Like Cell Invasion By Mir-183 Screened From The Peripheral Blood Of Pregnant Women With Preeclampsia

In recent years, microRNAs (miRNAs) have been shown to have critical regulatory roles in preeclamptic placentas. However, the effect and mechanisms of miRNAs on preeclamptic (PE) have not been well understood. This study aimed to test whether miR-183 could inhibit trophoblast-like cell invasion and reveal its underlying molecular mechanism. Differentially expressed miRNAs in the peripheral blood of preeclamptic compared with healthy pregnant women were validated using quantitative real-time polymerase chain reaction. The peripheral blood samples from patients with preeclamptic were analyzed for levels of a specific differentially expressed miRNA (miR-183). The effects of miR-183 were assessed by silencing and over-expressing the miRNA in vitro. Our study showed that miR-183 was specifically up-regulated in preeclampsia placentas. We found that overexpression of miR-183 inhibited the invasive capabilities of BeWo and JAR cells, whereas enhanced by knockdown of miR-183. We also confirmed that MMP9 as a direct target of miR-183 in BeWo cells by using the dual-luciferase assay. Furthermore, restoration of MMP9 expression in BeWo cells could partially reverse the cell invasion by miR-183 overexpression, indicating that miR-183 inhibits cytotrophoblast invasion via targeting MMP9. Ourstudy suggests miR-183 couldbe a promising therapeutic target for preeclampsia.