Effect Of Pterostilbene In Induction Of Apoptosis Through Down Regulation Of Caspase Activation In Ovarian Cancer Cells

Introduction: Pterostilbene is a natural dietary compound present in the blue berries. It is a methylated analog of resveratrol with increased bioavailability and lipophilicity compared to that of resveratrol. So, recent research has been into much focus on this compound. Objectives of the study: Thus the present study was designed with an aim to elucidate the mechanism of action of pterostilbene against ovarian cancer cells. Materials and methods: This study was conducted at SIMS, Lahore during 2016 to 2017 with the approval of ethical committee of hospital. The 5cc blood was drawn for the analysis of ROS. Reactive oxygen species was determined using fluorescent probe DCFDA. Pterostilbene-induced ROS generation was measured by DCF-DA. Results: The results showed that pterostilbene-induced ROS generation at 120%, 134% and 157% in 12, 24 and 48 h, respectively. Conclusion: The present study demonstrates for the first time that pterostilbene-induces apoptosis in ovarian cancer cell line, through ROS generation, mitochondrial depolarization, activation of caspase 9 and 3.