Analysis Of Levels Of Antioxidants And Catalases In The Blood With The Use Of Ropivacaine For Anesthesia

Introduction: Anaesthesia is a loss of sensation or a loss of consciousness and its mechanisms by which drugs can produce this state yet undetermined. Previous observations described how anaesthetic agents work at the molecular level and cause a loss of consciousness. Objectives of the study: The objective of this study was to determine if ropivacaine has any effects on the antioxidant defense system and in lipid peroxidation as Melanodialdehyde (MDA) of spinal fluid. Material and method: To measure the levels of lipid peroxidation, glutathione (GSH), superoxide dismutase (SOD) and the levels of catalases (CAT), samples were taken from the vein before anesthesia (0 min) and at 5, 15, and 60 min. after anesthesia. Results: The levels of MDA, SOD and GSH slightly increased at 5 min after spinal injection of ropivacaine and this increase continued throughout anesthesia (P<0.001). Catalases levels were decreased at 5 min after anesthesia (P<0.001) and this level not recovered after 60 min of anaesthesia. Conclusion: In conclusion, we found that spinal injection of ropivacaine increased free radical levels in spinal fluid which may be supported antioxidant environment of spinal fluid during anesthesia.