Elevated Expression Of Cyclophilin A In Human Periodontitis

This study elucidated the correlation between cyclophilin A (CypA) and inflammatory infiltrating cells in human periodontitis. Western blotting, immunohistochemistry, and immunofluorescence were performed to detect the expression level of CypA in gingival tissues of human periodontitis. Healthy gingival tissues were chosen as a control. The distribution of CD3(+), CD4(+), CD22(+), and CD68(+) infiltrating cells in the gingival tissues were also detected by immunohistochemistry. Western blotting analysis and immunohistochemistry revealed significantly increased expression of CypA in human periodontitis. NF-kappa B p-p65 and p-I kappa B alpha expression was detected to investigate NF-kappa B activation. Immunohistochemistry and immunofluorescence identified that the positive-expressed CypA was localized in the infiltrating cells. CD3(+), CD4(+), CD22(+), and CD68(+) cells all could be observed in the CypA-positive infiltrating cells. The NF-kappa B pathway was activated in human periodontitis. In conclusion, CypA is involved in leukocyte attraction in the periodontal inflammatory response. These efforts not only highlight the pathogenesis of human periodontitis, but also signify CypA as a potential target for anti-inflammatory therapeutics.