Lrrc4 Inhibits Mobility And Invasion Through The Sdf-1a/Cxcr4 Axis In U251 Cells

Glioblastomas are the most common and lethal human primary brain tumors, and are characterized by high invasiveness. SDF-1 alpha binds to CXCR-4 receptor and acts to modulate cell migration and proliferation. LRRC4 is a potential glioma suppressive gene. The reexpression of LRRC4 can significantly suppress glioblastomas U251 cells tumorigenesis in vivo and cell proliferation and invasion in vitro. In this study, we found that SDF-1 alpha could increase the invasion in U251 expressed CXCR4. The reintroduction of LRRC4 in U251 cells could inhibit the expression of CXCR4. LRRC4 also inhibited the adhesion ability of U251 to ECV304 as well as the mobility and invasion ability in vitro, which are mediated by SDF-1 alpha/CXCR4 axis. Further more, LRRC4 can greatly enhance GJIC of U251 cells. Thus, the reintroduction of LRRC4 in U251 cells could inhibit the expression of CXCR4 and the SDF-1 alpha/CXCR4 axis-mediated cells invasion in vitro.