Influence Of Glucagon-Like Peptide-2 On Intestinal Barrier Function And Immune Function And Changes In Tgf-Beta 1 Levels In Obstructive Jaundice Model Rats

Objective: The aim of the current study was to analyze the influence of glucagon-like peptide-2 (GLP-2) on intestinal barrier function (IBF) and immune function (IF), as well as changes in TGF-beta 1 levels, in obstructive jaundice (0J) model rats. Methods: A total of 60 healthy SD rats were selected and divided into the observation group (OG), including 20 intraperitoneally injected with GLP-2 as OJ models, the surgical control group (SCG), including 20 intraperitoneally injected with phosphate buffered solution (PBS) as OJ models, and the control group (CG), including 20 intraperitoneally injected with PBS and treated with laparotomy but not with common bile duct ligation (CBDL). These rats were killed in batches at 1 day, 3 days, 7 days, and 14 days after surgery, evaluating IBF and IF and measuring TGF-beta 1 levels. Results: (1) Proliferating cell nuclear antigen (PCNA) levels of CG were much higher than those of OG and SCG at 1 day, 3 days, and 7 days after surgery (P<0.05); (2) Caspase-3 levels of SCG were much higher than those of OG and CG at 3 days and 7 days after surgery (P<0.05); (3) Intestinal villus heights (IVH) of SCG levels were much lower than those of OG and CG at 1 day, 3 days, and 7 days after surgery (P<0.05); (4) CD4(+) and CD4(+)/CD8+ of OG and CG were higher than those of SCG and CD8(+) of OG and CG was lower than that of SCG at 1 day and 7 days after surgery (P<0.05); and (5) TGF-beta 1 levels of SCG were much higher than those of OG and CG at 7 days after surgery (P<0.05). Conclusion: GLP-2 can improve IBF and IF, enhancing TGF-beta 1 levels of OJ model rats.