Separation And Sizing Of A Virus Mixture Using Asymmetrical Flow Field-Flow Fractionation Coupled To Multi-Angle Light Scattering

Biogen and Postnova present data on the analysis of adeno-associated viruses (AAV) using Asymmetrical Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering detection (AF4-MALS). AAV are promising gene therapy delivery vehicles, whose efficacy may be negatively affected by the presence of viral aggregates. Due to its gentle separation and broad applicable size range, AF4-MALS is able to characterize AAV and their aggregates with high resolution and precision thereby overcoming the drawbacks that column-based chromatography techniques often face when dealing with samples larger than 50 nm in size.