Attenuation Of Obesity-Associated Oxidative Stress By Cucurbita Maxima Seed Oil In High Fat Diet-Induced Obese Rats

Obesity is a major risk factor for associated deleterious pathologies such as diabetes type 2, liver and heart disease. Many pieces of evidence of accumulated oxidative stress induced by obesity over the past two decades and play an important part in the growth of metabolic disorders. Based on this, the current study was designed to investigate the effect of Cucurbita maxima seed oil (CSO) on oxidative stress associated with high fat diet (HFD)-induced obesity in rats. The obese rats were supplemented with CSO (100 mg/kg body weight) for 30 days and determined the changes on hyperglycemic markers, tissue lipid profiles and oxidative stress markers such as TBARS, lipid peroxidation, protein carbonyls groups, glutathione, glutathione peroxidase, glutathione reductase, glutathione S-transferase, superoxide dismutase and catalase. Rats fed with HFD shown the significant increase of hyperglycemia and tissue lipid profiles and concomitantly decrease in the antioxidant enzymes and increased lipid peroxidation, protein carbonyl groups and TBARS. However, treatment with CSO for 30 days exhibited the noteworthy restoration of altered hyperglycaemic markers and lipid profiles. On the other hand, CSO increased the levels of antioxidant markers and concomitant decrease in the oxidative stress. In conclusion, these results suggested that CSO has the antioxidant potential and it might be used as therapeutic agent against oxidative stress related diseases such as hyperglycaemia and hyperlipidemia.