Expression Of Zp3 Glycoprotein In Bovine Oocytes Before And After Maturation And Their Interaction With Acrosomereacted Spermatozoa

The aim of the present study was to characterize ZP3 protein expression in bovine oocytes before and after maturation and short-term interaction of mature oocytes with acrosome-reacted spermatozoa. It was confirmed that ZP3 glycoprotein is expressed in bovine oocytes before and after maturation. A band with molecular weight of about 47 kDa corresponding to molecular weight of ZP3 protein was detected by Western blot both in immature and mature oocytes. Unlike them, no expression of ZP3 protein was found in mature oocytes after their interaction with acrosome-reacted spermatozoa.