The Elevated Blood Cd74 Mrna Associated With Prognosis In Septic Shock Patients

The hyperinflammation and immunosuppression were activated together in sepsis and septic shock patients by different inflammatory mediators or cytokines, such as CD74 and macrophage inhibitory factor (MIF), to kill bacteria and control infections in intensive care unit (ICU). In total, 88 cases (40 for sepsis and 28 for septic shock, 20 for healthy control) were enrolled in this study. Blood samples were collected at admission and Day 2, Day 4 after ICU admission. The level of serum CD74 was detected by ELISA kits. The blood CD74 mRNA was detected by real-time PCR. We found that serum CD74 levels were increased on day 2 after admission, and decreased on day 4 after admission to ICU in sepsis and septic shock patients. And blood CD74 mRNA kept the trend consistently with serum CD74, increasing at admission and Day 2, and then decreasing on day 4. But the level of serum CD74 and CD74 mRNA expression in both patients was markedly higher compared to healthy control cases. Meanwhile, we found that the concentration of serum CD74 was positive correlated with CD74 gene expression levels, at admission or on day 2, day 4. In consequence, the level of CD74 was higher in sepsis patients than in septic shock patients during ICU admission days, both for serum CD74 and blood CD74 mRNA. And multivariate analysis also revealed that blood CD74 mRNA was associated with prognostic factors for septic shock. These results implied that CD74 may play an important role in septic shock, and blood CD74 mRNA level was suitable to predict septic shock with disease severity after ICU admission.