Effects Of Chitosan Nanoparticle Carrier-Mediated Small-Interfering Rna Silencing Of Sumo-Specific Protease 1 On In Vitro Migration Of Breast Cancer Cells

Background: The aim of this study was to observe the effects of SENP1 silencing on the in vitro migration capacity of human breast cancer SK-BR-3 cells. Methods: They were transfected with chitosan nanoparticles (NPs) carrying SUMO-specific protease 1 small-interfering RNA (SENP1 siRNA). Chitosan-siRNA NPs were prepared by ionotropic gelation. Cells were transfected and divided into a chitosan-SENP1 siRNA NP (experimental) group, a Lipofectamine TM 2000 and SENP1 siRNA (positive control) group, a chitosan-siRNA NP (negative control) group and an empty chitosan NP (blank) group. The expressions of SENP1 in SK-BR-3 cells were detected by one-step reverse transcription-polymerase chain reaction and Western blot. The effects of SENP1 siRNA on in vitro migration capacity were evaluated by Transwell assay. One-way ANOVA and K-WH rank sum test were used for statistical analysis. Results: Spherical chitosan-siRNA NPs sized about 100 nm was successfully prepared. In the experimental group, SENP1 mRNA and protein expressions as well as in vitro cell migration were significantly inhibited compared with those in negative control and blank groups (P<0.01). Conclusion: Chitosan NP-mediated SENP1 siRNA effectively inhibited SENP1 expression in human breast cancer SK-BR-3 cells and their in vitro migration.