Differentiation Between Control Subjects And Patients With Chronic Spontaneous Urticaria Based On The Ability Of Anti-Ige Autoantibodies (Aabs) To Induce Fc Epsilon Ri Crosslinking, As Compared To Anti-Fc Epsilon Ri Alpha Aabs

Background: The reported prevalences of IgG autoantibodies (AAbs) to Fc epsilon RI alpha and IgE in sera from patients with chronic spontaneous urticaria (CSU) have varied, and these AAbs are also often observed in healthy control subjects. Regarding the histamine release activity of purified IgG from patients with CSU, the number of examined patients has been small. Thus, we sought to determine the prevalence and Fc epsilon RI crosslinking ability of these AAbs in a large number of patients with CSU and non-atopic control (NC) subjects.Methods: We compared the concentrations of anti-IgE and anti-Fc epsilon RI alpha AAbs and the abilities of these AAbs to cause Fc epsilon RI aggregation in patients with CSU (n = 134) and NC subjects (n = 55) using ELISA and an in vitro elicitation test, respectively.Results: The concentration of anti-IgE AAbs was significantly different between the NC subjects and the CSU patients (P < 0.0001, cutoff value: 0.558 mu g/mL), whereas the concentration of anti-Fc epsilon RI alpha AAbs was not. A significant difference in the duration of illness was noted between patients with lower and those with higher concentrations of anti-IgE AAbs relative to the cutoff value. The abilities of anti-IgE AAbs, but not anti-Fc epsilon RI alpha AAbs, to induce Fc epsilon RI crosslinking were significantly higher in CSU patients than in NC subjects (P = 0.0106).Conclusions: In the Japanese population of CSU patients studied, the ability of the anti-IgE AAbs to induce Fc epsilon RI crosslinking differed significantly between NC subjects and CSU patients, suggesting the involvement of anti-IgE AAbs in the pathogenesis of CSU in the Japanese population. Copyright (C) 2019, Japanese Society of Allergology. Production and hosting by Elsevier B.V.