Metabolic Engineering For The Production Of Lactic Acid From Xylose By The Thermoanaerobacterium Strain

Conversion of lignocelluloses to lactic acid can reduce the cost of the substrates, while the process requires strains capable of fermenting sugar mixtures of glucose and xylose. The screened strain of Thermoanaerobacterium aotearoense SCUT27 was successfully engineered by deleting the phosphate acetyltransferase and the acetate kinase. The resulting strain can grow on glucose and xylose to produce lactic acid under oxygen limited condition with the 1.3 and 2.5 folds increase of the quantity and yield of lactic acid production, respectively.