Hpv Oncogenes Regulated The Aberrant Expression Of Micrornas In Cervical Cancer

Abstract The high risk human papillomavirus (HPV) is the single most important etiological agent in cervical cancer. The E5, E6 and E7 genes in the early region of HPV genome are proved to have a significant impact on progression of cervical cancer. MicroRNA (miRNA) is a kind of short non-coding RNA which has been proven to play a role in tumor development. Previously, we have identified several differentially expressed miRNAs in cervical cell lines and clinical specimens, including 9 down-regulated and 2 up-regulated. Here we further investigate the direct relationship between the oncogenes (E5, E6 and E7) and the above miRNAs by overexpressing the oncogenes and silencing each single one separately. The result of the relative expression level of miRNAs showed some consistency that HPV E5, E6 and E7 could down-regulate the expression of miR-145, miR-195 and miR-142-3p. However, there were also discrepancy. For instance, E6 from HPV 16 and HPV 52 showed a significant down-regulation in the expression of miR-195, while E6 of other subtypes (HPV18, 31, 52) had no effect on the miR-195 expression level. Meanwhile, the oncogenetic effect of miR-142-3p has been examined by measuring cell proliferation. Further mechanism study of HPV/miR-142-3p pathway in the cervical cancer would also be investigated. The work could provide new insight in understanding the development of cervical cancer and potentially help to develop novel therapeutic approaches and bio-markers for cervical cancer. Citation Format: Yan Deng, Yi Song, Tao Tang, Chi Chiu Wang. HPV oncogenes regulated the aberrant expression of microRNAs in cervical cancer [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 5915.