Enzymatic Synthesis Of Colorimetric Substrates To Determine Alpha-2,3-And Alpha-2,6-Specific Neuraminidase Activity

Glycoconjugates containing either terminal alpha-2,3- or alpha-2,6-Neu5Ac-Gal disaccharides are found on cell surfaces of many animal glycans. Each linkage can be specifically recognized by lectins and enzymes such as neuraminidases. Here we describe a one-step enzymatic synthesis of two colorimetric substrates that allow for fast distinction of specific neuraminidase activity.