Differential Expression Of Nitrate Transport Protein, Putnar2.1, Of Puccinellia Tenuiflora In Response To Three Salinity Types

Puccinellia tenuiflora can survive on saline land and is an important halophyte. PutNAR2.1 was cloned from P. tenuiflora and mainly expressed in root PutNAR2.1 expression was significantly increased under the treatment of 300 mM NaCl,100 mM Na2CO3 or 150 mM NaHCO3 stress and reached the highest at 24 h in P. tenuiflora. Resistance to saline and alkali has been significantly improved when the PutNAR2.1 protein expression in E. coli. PutNAR2.1 transgenic yeast cells showed the more growth trend than control under NaCl, Na2CO3 or NaHCO3 stress. Seeds germination and seedlings growth of wild-type was affected heavily than the PutNAR2.1 transgenic Arabidopsis thaliana under NaCl, Na2CO3 and NaHCO3 stress. These results revealed that the expression of PutNAR2.1 was up-regulated by salt stress treatment. (C) 2020 Friends Science Publishers