Luteolin: A Novel Approach To Attenuating The Glaucoma Via Antioxidant Defense Mechanism

The aim of the current investigation was to explore the anti-glaucoma effect of leuteolin in the experimentally induced glaucoma. The New Zealand rabbit was used for the current experimental study. Intraocular pressure (IOP) percentage change was estimated after induction the dextrose induced acute glaucoma in rabbit. The rabbit was treated with the leuteolin (5, 10 and 20 mg/kg), acetazolamide (5 mg/kg) and normal saline, respectively. Another model such as prednisolone acetate and rabbits were treated with leuteolin, acetazolamide and normal saline, respectively for 2 weeks. We also evaluated the antioxidant effect of leuteolin via scrutinized the content of glutathione and glutamate in the experimental rabbits via using the ELISA assay. Histopathological observation of the ciliary bodies was also evaluated. Leuteolin significantly (P <= 0.001) inhibited the intraocular pressure in both acute and chronic glaucoma model, preserved glutamate and glutathione content. Histopathological studies showed the less inflammatory infiltration in the leuteolin and acetazolamide treated group rabbits as compared to the normal group rats. The result revealed that leuteolin proved the ocular hypotensive, neuroprotective and antioxidant effect, which consequently underscore its possible use as an anti-glaucoma drug with additional experimental study.