Activated Dna- And Rna-Duplexes Covalently Cross-Linked To Regulatory Proteins

A novel class of DNA- and RNA duplexes including trisubstituted pyrophosphate internucleotide groups (TSP) in sugar-phosphate backbone was employed in cross-linking to proteins. Substrates including TSP effectively bound covalently to nucleofilic aminoacids (Lys, His) of transcription factors HNF1 and NF-kB and to HIV-1 Tat protein without additional activation at physiological conditions.