An Optoelectronic Biomedical Method For Amphetamine Detection By Enzyme Multiplied Immunoassay Technique

Amphetamines are a class of substances with a stimulating effect on the central nervous system. These were first synthesided in 1887 by Lazar Edeleanu, a romanian chemist, in Germany, originally named phenylisopropylamine, but the stimulant effect of these was unknown until 1927, than it was resynthesized by Gordon Allens, who reported the sympathomimetic properties of amphetamines [1].Urine amphetamine measurements were performed on the Viva-ProE analyzer (SIEMENS, Germany). The light source is a 12V-20W iodine quartz lamp. The range of scannable wavelengths with its own selection of 8 filters is as follows (340, 415, 505, 546, 570, 600, 660, 700 nm). The bandwidth is 8 - 12 mn Photometric domain - Absorbance - 0.1 to 3.0. The authors present the results obtained by analyzing the urine samples from patients admitted in ICU II Toxicology with abuse substances overdose and working parameters of the Viva Pro-E System. Some of the results are presented. This analysis uses a threshold level of 300 ng / mL to distinguish positive and negative samples. The analysis provides only a preliminary analytical test result. A sample giving a change in the absorbance value (AA) equal to or even higher than the AA value of the Level 1 calibrator is interpreted as positive and the sample contains amphetamines or similar structural compounds or both.