Single-molecule simultaneous profiling of DNA methylation and DNA-protein interactions with Nanopore-DamID
biorxiv(2021)
摘要
DNA-protein interactions and cytosine methylation control eukaryotic gene expression. Here, we present an approach to simultaneously detect cytosine methylation and DNA-protein interactions from single molecules, through selective sequencing of adenine-labelled DNA. Applying this approach to LaminB1-associated heterochromatin domains, we identify strict CpG methylation maintenance at transcriptional start sites amidst a generalised relaxation of methylation, potentially to prevent ectopic and aberrant heterochromatic gene expression.
### Competing Interest Statement
The authors have declared no competing interest.
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