Hepatocyte androgen receptor in females mediates androgen-induced hepatocellular glucose mishandling and systemic insulin resistance
biorxiv(2021)
摘要
Androgen excess is one of the most common endocrine disorders of reproductive-aged women, affecting up to 20% of this population. Women with elevated androgens often exhibit hyperinsulinemia and insulin resistance. The mechanisms of how elevated androgens affect metabolic function are not clear. Hyperandrogenemia in a dihydrotestosterone (DHT)-treated female mouse model induces whole body insulin resistance possibly through activation of the hepatic androgen receptor (AR). We investigated the role of hepatocyte AR in hyperandrogenemia-induced metabolic dysfunction by using several approaches to delete hepatic AR via animal-, cell-, and clinical-based methodologies. We conditionally disrupted hepatocyte AR in female mice developmentally (LivARKO) or acutely by tail vein injection of an adeno-associated virus with a liver-specific promoter for Cre expression in ARfl/fl mice (adLivARKO). We observed normal metabolic function in littermate female Control (ARfl/fl) and LivARKO (ARfl/fl; Cre+/-) mice. Following chronic DHT treatment, female Control mice treated with DHT (Con-DHT) developed impaired glucose tolerance, pyruvate tolerance, and insulin tolerance, not observed in LivARKO mice treated with DHT (LivARKO-DHT). Further, during an euglycemic hyperinsulinemic clamp, the glucose infusion rate was improved in LivARKO-DHT mice compared to Con-DHT mice. Liver from LivARKO, and primary hepatocytes derived from LivARKO, and adLivARKO mice were protected from DHT-induced insulin resistance and increased gluconeogenesis. These data support a paradigm in which elevated androgens in females disrupt metabolic function via hepatic AR and insulin sensitivity was restored by deletion of hepatic AR.
### Competing Interest Statement
The authors have declared no competing interest.
* AR
: androgen receptor
PCOS
: polycystic ovary syndrome
DHT
: dihydrotestosterone
CAH
: congenital adrenal hyperplasia
HA
: hyperandrogenemia
Con
: control
Veh
: vehicle
LH
: luteinizing hormone
Foxo1
: forkhead box protein O1
PEPCK
: phosphoenolpyruvate carboxykinase
G6Pase
: glucose 6-phosphatase
I.P .
: intraperitoneal
DPM
: determine disintegrations per minute
BMI
: body mass index (BMI)
GTT
: glucose tolerance test
ITT
: insulin tolerance test
PTT
: pyruvate tolerance test
GSIS
: glucose stimulated insulin secretion
HGP
: hepatic glucose production
GIR
: glucose infusion rate.
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关键词
hepatocellular glucose,insulin resistance,systemic insulin resistance,androgen-induced
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