Fragmentation of Tissue-Resident Macrophages During Isolation Confounds Analysis of Single-Cell Preparations from Mouse Hematopoietic Tissues

Susan M. Millard,Ostyn Heng,Khatora S. Opperman,Anuj Sehgal,Katharine M. Irvine,Simranpreet Kaur,Cheyenne J. Sandrock,Andy C. Wu,Graham W. Magor,Lena Batoon,Andrew C. Perkins,Jacqueline E. Noll,Andrew C. W. Zannettino,David P. Sester,Jean-Pierre Levesque,David A. Hume,Liza J. Raggatt,Kim M. Summers,Allison R. Pettit

Cell Reports（2021）

Cited 37|Views38

Key words

macrophage,flow cytometry,imaging flow cytometry,RNA-seq,tissue-resident macrophage,bone marrow,spleen,lymph node,hematopoietic tissues,hematopoietic stem and progenitor cells

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