HaloTag9: an engineered protein tag to improve fluorophore performance
bioRxiv(2021)
摘要
HaloTag9 is an engineered variant of HaloTag7 with up to 40% higher brightness and increased fluorescence lifetime when labeled with fluorogenic rhodamines. Moreover, combining HaloTag9 with HaloTag7 and other fluorescent probes enabled live-cell multiplexing using a single fluorophore and the generation of a fluorescence lifetime-based biosensor. The increased brightness of HaloTag9 and its use in fluorescence lifetime multiplexing makes it a powerful tool for live-cell imaging.
### Competing Interest Statement
K.J. is inventor on patents filed by MPG and EPFL on fluorophores and labeling technologies.
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关键词
protein halotag9
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