Isolation and structure elucidation of two new cassane derivatives from the seed kernels of Caesalpinia sinensis .

Two new cassane-type derivatives (-), together with three known compounds (-), were isolated from the seed kernels of . Their structures were elucidated on the basis of interpretation of comprehensive spectroscopic data, including HRESIMS and 1D/2D NMR spectroscopy, and the absolute configuration were established by means of ECD calculation. Compound , possessing a 16-degradative cassane skeleton, was rarely encountered in cassane diterpenoids isolated from the genus . All compounds were evaluated for their anti-inflammatory activities against the overproduction of NO in LPS-stimulated RAW 264.7 macrophages, and compounds could inhibit production of NO at the concentration of 50 μM.