Genome-wide identification and transcriptional analyses of the R2R3-MYB gene family in wheat

MYB transcription factors (TFs) represents one of the largest TF families in plants. In this study, we performed genome-wide MYB-domain screening and identified a total of 997 MYBs in wheat (Triticum aestivum), among which 445 were 2-domain MYBs (R2R3-MYBs) that were clustered into 15 subgroups with varied conservation profiles. Homologous genes were highly conserved across the three subgenomes, with minor variations contributed by segmental duplications. Tandem and proximal gene duplications have contributed significantly to the expansion of the wheat Myb gene family. Furthermore, comprehensive transcriptome profiling of R2R3-Myb genes in 61 different tissue and time point samples revealed a clear pattern of temporal and spatial variations within six expression groups. The comprehensive genomic and transcriptional analyses provided valuable insights into the evolution and biological functions of R2R3-Myb genes in wheat. They would serve as a useful guide to further investigate the potential agronomic traits controlled by this large TF family. ### Competing Interest Statement The authors have declared no competing interest. * CS : Chinese Spring D : Days DPA : Days post-anthesis HAI : Hours after imbibition MYB : myeloblastosis NJ : Neighbor-Joining TAD : trans -acting domain TFs : Transcription factors TPM : Transcripts Per Kilobase Million WGD : Whole-genome duplication WS : Wheat subgroups