A Downsized and Optimised Intracellular Library-Derived Peptide Prevents Alpha-Synuclein Primary Nucleation and Toxicity Without Impacting Upon Lipid Binding

•Synucleinopathies are caused by aggregation of the protein alpha-Synuclein (aS)•The PreNAC region implicated in early onset PD serves as a peptide library template.•Ala scans result in a shorter modified peptide of significantly increased efficacy.•4654(N6A) prevents aS primary nucleation and toxicity but not lipid binding.