Single-Molecule RNA Imaging Using Mango II Arrays.
Methods in molecular biology (Clifton, N.J.)(2022)
摘要
In recent years, fluorogenic RNA aptamers, such as Spinach, Broccoli, Corn, Mango, Coral, and Pepper have gathered traction as an efficient alternative labeling strategy for background-free imaging of cellular RNAs. However, their application has been somewhat limited by relatively inefficient folding and fluorescent stability. With the recent advent of novel RNA-Mango variants which are improved in both fluorescence intensity and folding stability in tandem arrays, it is now possible to image RNAs with single-molecule sensitivity. Here we discuss the protocol for imaging Mango II tagged RNAs in both fixed and live cells.
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关键词
Fluorogenic RNA Aptamer,Live cell imaging,RNA dynamics,RNA-Mango,Single-Molecule Microscopy,Superresolution microscopy
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