Innovative Toolbox for the Quantification of Drosophila C Virus, Drosophila A Virus, and Nora Virus
Journal of Molecular Biology(2022)
摘要
•Novel ELISA-based endpoint dilution protocol to quantify infectious virus titer.•Negative strand specific RT-qPCR assays to quantify replicative viral RNA.•Simultaneous application of new methods in individual flies.
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关键词
virology,Drosophila melanogaster,virus quantification,RT-qPCR,ELISA
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