Production of n-caproate using food waste through thermophilic fermentation without addition of external electron donors.

The effectiveness of producing n-caproate from food waste without external electron donors (EDs) was investigated through batch and semi-continuous fermentation. The maximum concentration of n-caproate reached 10,226.28 mg COD/L during semi-continuous fermentation. The specificity for n-caproate was the highest at 40.19 ± 3.95%, and the soluble COD conversion rate of n-caproate reached up to 22.50 ± 1.09% at the end of batch fermentation. The production of n-caproate was coupled with the generation of lactate as an ED to facilitate chain elongation reactions. When lactate was used as the only substrate, n-butyrate (64.12 ± 20.11%) markedly dominated the products, instead of n-caproate (0.63 ± 0.07%). Microbial community analysis revealed that Caproiciproducens, Rummeliibacillus, and Clostridium_sensu_stricto_12 were the key genera related to n-caproate production. In addition to n-caproate, n-butyrate dominated the products in batch and semi-continuous fermentation with a maximum specificity of 47.59 ± 3.39%. Clostridium_sensu_stricto_7 was committed to producing n-butyrate from lactate.