Seroprevalence and isolation of Toxoplasma gondii in sheep intended for human consumption in Paraíba, northeastern Brazil

Toxoplasma gondii is a zoonotic parasite capable of infecting almost all warm-blooded animals. Currently, studies assessing the viability of cysts present in sheep meat are still scarce, especially in the northeastern region of Brazil. Therefore, the aim of this study was to evaluate the seroprevalence of anti- Toxoplasma gondii antibodies, isolate the parasite from tissue samples, and determine factors associated with infection of sheep intended for human consumption in the state of Paraíba, northeastern Brazil. Serum samples from 205 sheep slaughtered in Paraíba were tested using the indirect fluorescence antibody test (IFAT ≥ 64) for the presence of anti- T. gondii antibodies. Among the animals sampled, 30.7% (63/205) were seropositive, with antibody titers ranging from 1:64 to 1:16384. Sixty-one bioassays were performed in mice, and an isolation rate of 47.5% (29/61) was obtained. The infection was lethal to at least one infected mouse in 24.1% (7/29) of the bioassays. The mean length of survival of infected mice was 36.4 days. Females (OR = 3.183; CI = [1.452–6.976]), extensive breeding system (OR = 2.612; CI = [1.120–6.094]), and purebred animals (OR = 2.157; CI = [1.009–4.612]) were considered to be factors associated with infection. These results revealed that the prevalence of anti- T. gondii antibodies and the percentage of viable cysts recovered from sheep destined for human consumption in Paraíba, northeastern Brazil, were high.