Cell Wall Galactofuranan And Pyruvate-Containing Galactomannan In The Cell Walls Of Clavibacter Strains

The cell wall glycopolymer structures of plant-associated strains Clavibacter sp. VKM Ac-1371, Clavibacter sp. VKM Ac-1372 and Clavibacter sp. VKM Ac-1374, members of three putative new species (family Microbacteriaceae, class Actinobacteria) were studied. Each strain was found to contain two glycopolymers, neutral and acidic ones. The main chain of neutral polymer, identical in all three strains, is (1 -> 6)-linked beta-D-galactofuranan with every second galactofuranose residue substituted at position 2 by side disaccharide, alpha-D-Manp-(1 -> 2)-alpha-D-Ribf-(1 ->. The second, acidic polymer, is pyruvate-containing galactomannan with the repeating unit, -> 3)-alpha-D-Galp-(1 -> 3)-alpha-D-[4,6-S-Pyr]-Manp-(1 -> 3)-alpha-D-Manp-(1 ->. Reducing mannopyranose residues of the acidic polysaccharides repeating unit from strains VKM Ac-1372 and VKM Ac-1374 bear O-acetyl residues additionally. The cell wall glycopolymer structures were established by chemical and NMR spectroscopic methods with using one-and two-dimensional techniques H-1,H-1 COSY, TOCSY, ROESY and H-1,C-13 HSQC, HMBC. The results obtained provide new data on diversity of the bacterial cell wall glycopolymers and may prove valuable for microbial taxonomy and insight into the molecular mechanisms of interactions between bacteria and plants and also of bacterial adaptation to survival in desert systems.