Pedobacter riviphilus sp. nov., isolated from stream sediment

A Gram- stain- negative, aerobic, motile by gliding, rod- shaped and pink- coloured bacterium, designated strain SW- 16T, was isolated from the sediment of small stream in the Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SW-16T formed a lineage within the genus Pedobacter of the family Sphingobacteriaceae. Phylogenetic analysis also showed that strain SW-16T was most closely related to Pedobacter soli 15-51T (98.0% 16S rRNA gene sequence similarity), Pedobacter humicola R135T (97.5%), was observed at 10-37 degrees C (optimum at 30 degrees C), pH 6-8 (optimum at pH 7) and with 0-2.0 % NaCl (optimum at 0%). The major fatty acids of the bacterial strain were iso- C15:0, iso-C17: 0 3- OH and summed feature 3 (iso- C15: 0 2- OH and/or C16:1 omega 7c). The predominant respiratory quinone was menaquinone- 7 and the major polar lipids were phosphatidylethanolamine, two unidentified amino lipids, one unidentified phospholipid and three unidentified lipids. The genome size of strain SW- 16T was 5.8 Mbp and the G+C content was 38.5 mol%. Based on the results of phenotypic, genomic and phylogenetic analyses, strain SW- 16T represents a novel species of the genus Pedobacter, for which the name Pedobacter riviphilus sp. nov. is proposed. The type strain is SW- 16T (=KEMB 1602-396T=KCTC 82079T=JCM 34181T).