Single-crystal ordered macroporous metal-organic framework as support for molecularly imprinted polymers and their integration in membrane formant for the specific recognition of zearalenone

Zearalenone is a fungal contaminant that is widely present in grains. Here, a novel molecularly imprinted membrane based on SOM-ZIF-8 was developed for the rapid and highly selective identification of zearalenone in grain samples. The molecularly imprinted membrane was prepared using polyvinylidene fluoride, cyclododecyl 2,4-dihydroxybenzoate as a template and SOM-ZIF-8 as a carrier. The factors influencing the extraction of zearalenone using this membrane, including the solution pH, extraction time, elution solvent, elution time, and elution volume, were studied in detail. The optimized conditions were 5 mL of sample solution at pH 6, extraction time of 45 min, 4 mL of acetonitrile:methanol = 9:1 as elution solvent, and elution time of 20 min. This method displayed a good linear range of 12-120 ng/g (R-2 = 0.998) with the limits of detection and quantification of this method are 1.7 and 5.5 ng/g, respectively. In addition, the membrane was used to selectively identify zearalenone in grain samples with percent recoveries ranging from 87.9 to 101.0% and relative standard deviation of less than 6.6%. Overall, this study presents a simple and effective chromatographic pretreatment method for detecting zearalenone in food samples.