Microvirga pudoricolor sp. nov., and Microvirga alba sp. nov., isolated from soil in South Korea

Two novel Gram-stain-negative, aerobic, rod-shaped, circular, convex, light-pink and white-colored bacterial strains BT291 T and BT350 T were isolated from soil collected in Uijeongbu city (37° 44′ 55″ N, 127° 2′ 20″ E) and Jeju island (33° 22′ 48″ N, 126° 31′ 48″ E), respectively, South Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that each of the strains BT291 T and BT350 T belong to a distinct lineages within the genus Microvirga (family Methylobacteriaceae , order Rhizobiales , class Alpha Proteobacteria , phylum Proteobacteria , kingdom Bacteria ). The 16S rRNA gene sequence similarity between the two strains BT291 T and BT350 T was 97.4%. The two strains were found to have the same quinone system, with Q-10 as the major respiratory quinone. The major polar lipids of strains BT291 T and BT350 T were phosphatidylethanolamine (PE), diphosphatydilglycerol (DPG), phosphatidylcholine (PC) and phosphatidylglycerol (PG). The major cellular fatty acids of strain BT291 T were C 18:1 ω 7 c (58.2%) and cyclo-C 19:0 ω 8 c (25.7%). The major cellular fatty acids of strain BT350 T were C 18:1 ω 7 c (38.5%) and cyclo-C 19:0 ω 8 c (27.7%). Based on the polyphasic analysis (phylogenetic, chemotaxonomic and biochemical), strains BT291 T and BT350 T can be suggested as two novel bacterial species within the genus Microvirga and the proposed names are Microvirga pudoricolor and Microvirga alba , respectively. The type strain of Microvirga pudoricolor is BT291 T (= KCTC 72368 T = NBRC 114845 T ) and the type strain of Microvirga alba is BT350 T (= KCTC 72385 T = NBRC 114848 T ).